Synthetic metabolic pathways for carbon dioxide fixation

Category Science

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The Max Planck Institute have created a synthetic metabolic pathway that takes carbon dioxide into formaldehyde, offering a carbon-neutral method for producing valuable materials, such as pharmaceuticals and active ingredients. The process is driven by energy, such as electricity from solar modules, and can be used to supply other highly reactive substances with no toxic effects.

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Scientists at the Max Planck Institute have created a synthetic metabolic pathway that converts carbon dioxide into formaldehyde via formic acid, offering a carbon-neutral method for producing valuable materials.

In view of rising greenhouse gas emissions, carbon capture, the sequestration of carbon dioxide from large emission sources, is an urgent issue. In nature, carbon dioxide assimilation has been taking place for millions of years, but its capacity is far from sufficient to compensate for man-made emissions.

Formaldehyde has a toxic effect on living organisms and is a potential health hazard

Researchers led by Tobias Erb at the Max Planck Institute for Terrestrial Microbiology are using nature’s toolbox to develop new ways of carbon dioxide fixation. They have now succeeded in developing an artificial metabolic pathway that produces the highly reactive formaldehyde from formic acid, a possible intermediate product of artificial photosynthesis. Formaldehyde could be fed directly into several metabolic pathways to form other valuable substances without any toxic effects. As in the natural process, two primary components are required: Energy and carbon. The former can be provided not only by direct sunlight but also by electricity — for example from solar modules.

The metabolic pathway created requires ATP as energy source and can also be driven by electricity, such as from solar modules

Within the added-value chain, the carbon source is variable. carbon dioxide is not the only option here, all monocarbons (C1 building blocks) come into question: carbon monoxide, formic acid, formaldehyde, methanol, and methane. However, almost all of these substances are highly toxic — either to living organisms (carbon monoxide, formaldehyde, methanol) or to the planet (methane as a greenhouse gas). Only formic acid, when neutralized to its base formate, is tolerated by many microorganisms in high concentrations.

Formic acid is a naturally produced acid in the stomach of ants

"Formic acid is a very promising carbon source," emphasizes Maren Nattermann, first author of the study. "But converting it to formaldehyde in the test tube is quite energy-intensive." This is because the salt of formic acid, formate, cannot be converted easily into formaldehyde. "There’s a serious chemical barrier between the two molecules that we have to bridge with biochemical energy — ATP — before we can perform the actual reaction.

Formaldehyde can be used in many biochemical techniques, such as PCR and gel electrophoresis

The researcher’s goal was to find a more economical way. After all, the less energy it takes to feed carbon into metabolism, the more energy remains to drive growth or production. But such a path does not exist in nature. "It takes some creativity to discover so-called promiscuous enzymes with multiple functions," says Tobias Erb. "However, the discovery of candidate enzymes is only the beginning. We’re talking about reactions that you can count along with since they’re so slow — in some cases, less than one reaction per second per enzyme. Natural reactions can happen a thousand times faster." This is where synthetic biochemistry comes in, says Maren Nattermann: "If you know an enzyme’s structure and mechanism, you know how to tweak it to perform this reaction more efficiently." .

The enzymes involved in the metabolic pathway can be ‘tweaked’ to increase efficiency

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