CReATiNG Technique Revolutionizes Synthetic Biology

Thursday - December 21 2023, 02:48 UTC - 11 months ago

USC Dornsife’s CReATiNG technique is a groundbreaking new method that revolutionizes synthetic biology by making the cost-effective construction of synthetic chromosomes possible. It could significantly advance genetic engineering and enable a wide range of advances in medicine, biotechnology, biofuel production, and even space exploration.

USC Dornsife’s CReATiNG technique revolutionizes synthetic biology by facilitating the cost-effective construction of synthetic chromosomes, promising significant advancements in various scientific and medical fields. A groundbreaking new technique invented by researchers at the USC Dornsife College of Letters, Arts and Science may revolutionize the field of synthetic biology. Known as CReATiNG (Cloning Reprogramming and Assembling Tiled Natural Genomic DNA), the method offers a simpler and more cost-effective approach to constructing synthetic chromosomes. It could significantly advance genetic engineering and enable a wide range of advances in medicine, biotechnology, biofuel production, and even space exploration.

CReATiNG works by cloning and reassembling natural DNA segments from yeast, allowing scientists to create synthetic chromosomes that can replace their native counterparts in cells. The innovative technique enables researchers to combine chromosomes between different yeast strains and species, change chromosome structures, and delete multiple genes simultaneously.

Lead researcher Ian Ehrenreich, professor of biological sciences at USC Dornsife, said the method is a major improvement over current technology. “With CReATiNG, we can genetically reprogram organisms in complex ways previously deemed impossible, even with new tools like CRISPR,” he said. “This opens up a world of possibilities in synthetic biology, enhancing our fundamental understanding of life and paving the way for groundbreaking applications.” .

The study will be published today (December 20) in the journal Nature Communications.

The field of synthetic biology has emerged as a way for scientists to take control of living cells, such as yeast and bacteria, to better understand how they work and to enable them to produce useful compounds, such as new medicines.

“Over the last decade or so, a new form of synthetic biology has emerged called synthetic genomics, which involves synthesizing whole chromosomes or entire genomes of organisms,” Ehrenreich said. “The thing about most synthetic genomics research is that it involves building chromosomes or genomes from scratch using chemically synthesized DNA pieces. This is a ton of work and extremely expensive.” .

However, there have been no alternatives — until now. “CReATiNG offers an opportunity to use natural pieces of DNA as parts to assemble whole chromosomes,” said Agilent postdoctoral fellow Alessandro Coradini, who was study first author.

The method makes advanced genetic research more accessible by significantly lowering costs and technical barriers so scientists can unlock new solutions to some of the most pressing challenges in science and medicine today.

The findings are particularly significant for their potential applications in biotechnology and medicine. CReATiNG could lead to more efficient production of pharmaceuticals and biofuels, aid in the development of cell therapies for diseases like cancer, and pave the way to methods of environmental bioremediation, such as creating bacteria that consume pCO2 or manage oil spills.